Convert cytometer fluorescence signals to calibrated reference units using your own bead data
Enter the known reference values (e.g. MESF) and observed MFI for each bead population. The first row is used as background and subtracted from subsequent populations.
| Pop | Known MESF | Observed MFI |
|---|
The regression model fit to your bead data. Verify R² is high before proceeding to convert samples.
Enter bead data to see the equation.
Enter your sample MFI values to convert them to calibrated reference units (e.g. MESF) using the bead regression.
| # | Sample MFI | Background MFI | Reference Values |
|---|